![]() When compared to sonication, xTractor Buffer extraction yielded more protein content from the same size sample. ![]() This cell lysis protocol is a highly efficient means of extracting proteins from any cells in order to obtain increased protein purity and yield. To save time, the resulting lysate can be loaded directly on a TALON CellThru Column without centrifugation or filtration. After extraction, simply centrifuge or filter the lysate and load it on any affinity resin column to isolate your tagged proteins. The final lysate has no visible cell fragments or precipitates. Just resuspend the cell pellet in the cell lysis buffer (1:20 w/v) and mix gently for 10 minutes, reducing the risk of proteolysis. ![]() xTractor Buffer has been optimized for extraction of his-tagged proteins, so it is compatible with all IMAC resins. This cell lysis reagent provides fast and efficient (10 min) lysis using mild, non-denaturing conditions. XTractor Buffer is the most flexible and efficient cell lysis buffer for protein extraction from bacterial, yeast, mammalian, and baculovirus-infected cells.
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